Got the transmutation to work in one direction. Going to use a new primer to run it the other way!
Primer!
Got colonies to grow! Time to sequence…
Back in the lab (Phase VII). Transmutation is going well. It may take a few tries, but it looks like the path to characterizing the protein – EGCG complex is getting shorter!
Today, I learned that I earned a First Place at the Georgia State Science Fair! (phase VII has already been underway…)
Preparing the Plasmid for Sequencing…
Nanodrop Spectrophotometer
Testing the concentration of the plasmid. Got a great yield!
Arrival Of The Custom Plasmid!
I finally received my custom designed plasmid yesterday.
Now I can get to producing the protein that helps cancer to proliferate. . .
and then give it what it needs to function, with a little modification. . .
Modifying EGCG In Preparation for the Final Step. . .
Using the Biolayer Interferometer with EGCG and a Peptide. Physics and Biochemistry collide. . .
Preparing peptides and EGCG for NMR analysis on a brand new 600Mhz NMR Spectrometer.
EGCG, A PATHWAY TOWARD A CURE FOR CANCER 